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Appl Environ Microbiol. 1972 May; 23(5): 994-997
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Tolerance of Staphylococcal Thermonuclease to Stress

Section of Infectious and Immunologic Diseases, Department of Internal Medicine, School of Medicine, University of California, Davis, California 95616
Department of Epidemiology and Preventive Medicine, School of Veterinary Medicine, University of California, Davis, California 95616

ABSTRACT

Remarkable tolerance to prolonged heating, prolonged storage, and bacterial proliferation was exhibited by staphylococcal thermonuclease in foods and broth. A purified enzyme preparation added to Brain Heart Infusion broth was unaffected by the growth of five bacterial species. Minimal inactivation was effected by Bacillus subtilis. Optimal growth of Streptococcus faecalis var. liquifaciens caused extensive inactivation of thermonuclease. However, storage at room temperature or the addition of 5% NaCl caused only minimal inactivation.