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Appl Environ Microbiol. 1972 May; 23(5): 998-1000
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Susceptibility of Phytopathogenic Bacteria to Wheat Purothionins In Vitro

R. Fernandez De Caleya, Blanca Gonzalez-Pascual, F. García-Olmedo and Pilar Carbonero

Departamento de Bioquímica, E.T.S. de Ingenieros Agrónomos, Universidad Politécnica, Madrid-3, Spain
Departamento de Bioquímica, Microbiologia y Fisiologia, Instituto Nacional de Investigaciones Agrarias, Madrid-3, Spain

ABSTRACT

Purothionins are basic polypeptides with antimicrobial properties that are present in the endosperm of wheat and other cereal species. Susceptibility to wheat purothionins among phytopathogenic bacteria of the genera Pseudomonas, Xanthomonas, Agrobacterium, Erwinia, and Corynebacterium has been investigated. Sensitive strains have been found in all of these genera except Agrobacterium (the only strain of A. tumefaciens available proved to be resistant). Minimal inhibitory concentrations (MIC) with partially purified crude purothionins ranged from 1 µg/ml for C. sepedonicum (C.5) to 540 µg/ml for E. amylovora (E.3). Minimal bactericidal concentrations (MBC) were not higher than twice the MIC value, except for C. poinsettiae (C.4) (MBC/MIC = 8). Purothionins {alpha} and ß, obtained by carboxymethyl-cellulose column chromatography, were tested against P. solanacearum (P.2) and X. phaseoli (X.2); {alpha} purothionin was more active than ß against X.2, and ß more active than {alpha} against P.2. This suggests a relationship between polypeptide sequence and specificity of action.

Appl Environ Microbiol. 1972 May; 23(5): 998-1000
Copyright © 1972 American Society for Microbiology. All Rights Reserved.





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Copyright © 1972 by the American Society for Microbiology. All rights reserved.