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Appl Environ Microbiol. 1972 June; 23(6): 1097-1100
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Agar Block Technique for Identification of Mycoplasmas by Use of Fluorescent Antibody¹

^a Department of Clinical Pathology, School of Veterinary Medicine, University of California, Davis, California 95616

ABSTRACT

A procedure for staining mycoplasmata colonies directly on agar blocks for examination by fluorescent microscopy is described. Areas of the agar surface appropriate for staining were demarcated by use of Lucite cylinders. Direct fluorescent-antibody staining was superior to indirect staining. The technique was very useful for determining whether cultures were mixed and for identification of mycoplasmas in either pure or mixed cultures.

² Present address: Veterinary Resources Branch, Division of Research Service, National Institutes of Health, Bethesda, Md. 20014.

¹ Based upon a thesis presented by E. J. Baas to the faculty of the Graduate School of the University of California at Davis, California, in partial fulfillment of the requirements for the Ph.D. degree.