This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Guskey, L. E. Articles by Wolff, D. A. Search for Related Content PubMed Articles by Guskey, L. E. Articles by Wolff, D. A. Agricola Articles by Guskey, L. E. Articles by Wolff, D. A.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1972 July; 24(1): 13-17
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Concentration and Purification of Poliovirus by Ultrafiltration and Isopycnic Centrifugation¹

^a Department of Microbiology, The Ohio State University, Columbus, Ohio 43210

ABSTRACT

A method is described by which poliovirus can be rapidly and simply concentrated by the use of a Diaflo XM-50 ultrafilter membrane. Freon-extracted ultrafilter concentrates banded in CsCl provided a 1,724-fold volumetric concentration of poliovirus. During concentration, trypsin-digested cellular material can pass through the ultrafilter membrane, thus providing a versatile means of degrading and eliminating extraneous contaminating proteins. The ultrafilter concentration system is compared with the CsCl cushion system of poliovirus concentration, and both systems are further compared by banding virus and virus capsid material in CsCl by use of isopycnic centrifugation.

² Present address: Department of Biology, Ciba-Geigy, Basel, Switzerland.

¹ Presented in part at the 69th Annual Meeting of the American Society for Microbiology, Detroit, Mich., 1969.