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Appl Environ Microbiol. 1972 August; 24(2): 175-178
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Stomaching: a New Concept in Bacteriological Sample Preparation

Unilever Research Laboratory Colworth/Welwyn, Colworth House, Sharnbrook, Bedford, England

ABSTRACT

An entirely new mixing device, particularly suitable for preparing bacterial suspensions from foods, fabrics, swabs, and other fairly soft materials, has been developed. With this technique the sample and diluent are put into an inexpensive, sterile plastic bag which is vigorously pounded on its outer surfaces by paddles when placed inside the machine. The resulting compression and shearing forces effectively remove even deep-seated bacteria. After samples are taken for analysis the bag and its remaining contents are thrown away. Labor involved in cleaning and sterilizing reusable homogenizer cups or probes is eliminated, and the device is immediately ready for reuse. Running costs are thus drastically reduced, compared with conventional homogenizers. Additional advantages of this device, which is simple and inexpensive to manufacture, are low noise level, negligible temperature rise, and the small storage space required for bags.