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Appl Environ Microbiol. 1972 August; 24(2): 270-274
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Use of Polyelectrolytes and Electron Microscopy for Detection of Viruses from Stool

¹ Department of Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada

ABSTRACT

Insoluble polyelectrolytes (PE60) were used for the concentration of viruses from stool specimens, confirming the results of Wallis et al. (1969). Ten percent suspensions inoculated with poliovirus type 3 were used in these experiments. A small number of stool specimens from patients naturally infected with enteroviruses were also tested. Preferential adsorption of viruses to PE60 was maximum at a pH range of 4.5 to 6.0. The elution of the adsorbed viruses was optimal at pH 8.5. Other parameters were also investigated. Electron microscopy was used successfully to detect the eluted viruses.