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Appl Environ Microbiol. 1972 August; 24(2): 283-285
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Demonstration of Rhodanese Activity in Polyacrylamide Gels

J. R. Murphy, J. Frankenfeld, J. Calvert and R. C. Tilton

1 University of Connecticut Health Center, Hartford, Connecticut 06105

ABSTRACT

Rhodanese activity from crude extracts of Thiobacillus sp. strain IV-85 was demonstrated in polyacrylamide gels after incubation in the reaction mixture by staining with dichloroindophenol in the presence of methylphenazonium methosulfate. The sensitivity of the staining system was found to be 8 x 10-7 moles of sulfite.

Appl Environ Microbiol. 1972 August; 24(2): 283-285
Copyright © 1972 American Society for Microbiology. All Rights Reserved.





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Copyright © 1972 by the American Society for Microbiology. All rights reserved.