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Appl Environ Microbiol. 1972 September; 24(3): 437-439
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Occurrence of Aflatoxins and Aflatoxin-Producing Strains of Aspergillus spp. in Soybeans ¹

Department of Botany, University of Maryland, College Park, Maryland 20742
Department of Agronomy, University of Maryland, College Park, Maryland 20742

ABSTRACT

Above average rainfall in Maryland during August, September, and October 1971 resulted in heavy mold growth in soybeans while still in the field. Of 28 samples of soybean seed, aflatoxins were found in 14, 2 of which had been used in poultry feed. Aflatoxins were identified by thin-layer chromatography, spectrophotometry, and chicken embryo bioassay. Aspergillus spp. were isolated from 11 samples, and 5 of these isolates produced aflatoxins when grown in liquid culture.

¹ Scientific Article A1768, Contribution 4556 of the Maryland Agricultural Experiment Station.