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Appl Environ Microbiol. 1972 November; 24(5): 684-690
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Identification of T-2 Toxin in Moldy Corn Associated with a Lethal Toxicosis in Dairy Cattle

Ih-Chang Hsu, E. B. Smalley, F. M. Strong and William E. Ribelin

Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706
Department of Plant Pathology, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706
Department of Veterinary Science, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

Over a 5-month period during the winter of 1970-71, 20% of the lactating Holstein cows in a Wisconsin dairy herd died after prolonged ingestion of a diet containing 60% moldy corn infested with Fusarium tricinctum (2 x 105 propagules per g of moldy corn). Ethyl acetate extracts of the ground dried corn induced severe dermal reactions when applied to the skin of shaved 60-g albino rats and killed four of five 100-g rats that were force fed 1 ml in 2 ml of pure corn oil. T-2 toxin (3-hydroxy-4, 15-diacetoxy-8-[3-methylbutyryloxy]-12, 13-epoxy-{Delta}9-trichothecene) at concentrations of 2 mg per kg of dry corn was identified in purified extracts of the moldy corn by means of gas-liquid chromatography and mass spectrometry. This concentration of T-2 toxin in the moldy feed and the nature of the toxic effects observed strongly suggest a major causal relationship.

Appl Environ Microbiol. 1972 November; 24(5): 684-690
Copyright © 1972 American Society for Microbiology. All Rights Reserved.





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