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Appl Environ Microbiol. 1972 November; 24(5): 742-749
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Simplified Radioimmunoassay for Diagnostic Serology

Center for Disease Control, Health Services and Mental Health Administration, U. S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333

ABSTRACT

A simplified, indirect radioimmunoassay is described for Escherichia coli, vaccinia virus, and herpesvirus. The antigens were affixed to glass cover slips; thus both the primary and secondary reactions take place on the cover slips, and the unbound antiserum is easily separated from the bound antiserum by rinsing. Rabbit or human immune sera were reacted with the antigens, and the primary immune complex was quantitated by a secondary reaction with ¹²⁵I-indicator globulin (anti-rabbit or anti-human). A direct relationship between the antiserum concentration and the ¹²⁵I absorption was established. Variations in titers were detectable, and the titers were comparable to complement fixation titers. Homologous and heterologous reactions were distinguishable. The method affords an objective, quantitative, and qualitative evaluation of antibody, and results are reproducible.