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Appl Environ Microbiol. 1972 December; 24(6): 995-996
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Mutagenesis and Enrichment of Auxotrophs in Penicillium chrysogenum1

Prakash S. Masurekar, Marc P. Kahgan and Arnold L. Demain

a Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

ABSTRACT

An efficient method for isolation of auxotrophs of Penicillium chyrysogenum involving mutagenesis with ethyl methanesulfonate followed by enrichment with sodium pentachlorophenate was developed. The auxotroph frequencies obtained were 30 to 40%.

FOOTNOTES

1 Contribution no. 2025 from the Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Mass. 02139.

Appl Environ Microbiol. 1972 December; 24(6): 995-996
Copyright © 1972 American Society for Microbiology. All Rights Reserved.





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Copyright © 1972 by the American Society for Microbiology. All rights reserved.