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Appl Environ Microbiol. 1973 February; 25(2): 216-221
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Simple Method for Culturing Anaerobes

Clinical Microbiology Laboratory, University of California, San Diego, California 92103
Division of Clinical Pathology, University of California, San Diego, California 92103
Department of Pathology, University of California, San Diego, California 92103
Division of Infectious Disease, University of California, San Diego, California 92103
Department of Medicine, University of California, San Diego, California 92103

ABSTRACT

A simple, effective method is needed for growing obligate anaerobes in the clinical laboratory. This report describes a pre-reduced anaerobic bottle that can be taken to the bedside for direct inoculation, provides a flat agar surface for evaluation of number and morphology of colonies, and can be incubated in conventional bacteriological incubators. Each anaerobic culture set consisted of two bottles containing brain heart infusion agar and CO₂. Gentamicin sulfate (50 µg/ml) was added to one of these to inhibit facultative enteric bacilli. Comparison of the anaerobic bottles with an identical aerobic bottle which was also routinely inoculated permitted early identification of anaerobic colonies. Representative species of most anaerobic genera of proven pathogenicity for man have been isolated from this system during 10 months of routine use.