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Appl Environ Microbiol. 1973 February; 25(2): 244-252
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Spiral Plate Method for Bacterial Determination

Division of Microbiology, Food and Drug Administration, Cincinnati, Ohio 45226

ABSTRACT

A method is described for determining the number of bacteria in a solution by the use of a machine which deposits a known volume of sample on a rotating agar plate in an ever decreasing amount in the form of an Archimedes spiral. After the sample is incubated, different colony densities are apparent on the surface of the plate. A modified counting grid is described which relates area of the plate of volume of sample. By counting an appropriate area of the plate, the number of bacteria in the sample is estimated. This method was compared to the pour plate procedure with the use of pure and mixed cultures in water and milk. The results did not demonstrate a significant difference in variance between duplicates at the = 0.01 level when concentrations of 600 to 12 x 10⁵ bacteria per ml were used, but the spiral plate method gave counts that were higher than counts obtained by the pour plate method. The time and materials required for this method are substantially less than those required for the conventional aerobic pour plate procedure.