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Appl Environ Microbiol. 1973 February; 25(2): 270-274
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Isotopic Labeling of Embryo, Yolk Sac, and Intracellular Parasites of the Embryonated Hen's Egg by Yolk Replacement

Department of Microbiology, University of Virginia Medical School, Charlottesville, Virginia 22903

ABSTRACT

A technique is described which allows the replacement of 50% of the yolk of the embryonated hen's egg with large volumes of diverse but chemically defined solutions. By using an electrosurgical unit and a polyethylene tunnel, the procedure was performed on eggs from days 3 through 7 with greater than 90% surgical success and viability for the short term. More than 50% of the eggs replaced showed viability for 2 weeks, and a significant proportion went full term. ³²PO₄ and amino acids (³H and ¹⁴C) added to the replaced eggs were incorporated into the macromolecules of the embryo and yolk sac as well as into parasitic rickettsiae cultivated in the replaced eggs. The incorporated ³²PO₄ was shown to be assimilated into a variety of biochemical species.