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Appl Environ Microbiol. 1973 March; 25(3): 346-353
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Production and Purification of Large Amounts of Rous Sarcoma Virus

Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710
University Laboratories, Inc., Highland Park, New Jersey 08904

ABSTRACT

Procedures are described for production and purification of large amounts of Rous sarcoma virus. The virus was produced by Rous sarcoma virus-transformed chicken embryo fibroblasts in roller culture which produced up to 6 mg of virus per day per liter of supernatant fluid. Various methods of concentrating virus were evaluated; pelleting yielded the best results in terms of recovery of infectious virus. Purification was achieved by means of successive velocity and equilibrium density centrifugation by using sucrose solutions made in low-salt buffer. A rapid method for the optical density measurement of virus concentration was also developed.