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Appl Environ Microbiol. 1973 March; 25(3): 458-460
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Comparison of the Direct Agglutination and Indirect Hemagglutination Tests in the Determination of Blood Serum Titers to Escherichia coli Organisms¹

^a Department of Veterinary Science, North Dakota State University, Fargo, North Dakota 58102

ABSTRACT

A comparison of the direct agglutination test and the indirect hemagglutination test for the detection of blood serum antibodies to Escherichia coli organisms indicated that these serological tests were comparable. In some instances the indirect hemagglutination test provided higher endpoint readings. Preparation of the antigens for the indirect hemagglutination test was more time consuming than for the direct agglutination test. Crude extract and purified polysaccharides were comparable as red blood cell sensitizing agents.

¹ Published with the approval of the director of the North Dakota Agricultural Experiment Station as Science Series 379.