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Appl Environ Microbiol. 1973 June; 25(6): 880-884
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Preparation of Solid-Phase Immunosorbents by Coupling Human Serum Proteins to Cyanogen Bromide-Activated Agarose

J. D. Sipe and F. V. Schaefer

1 Electro-Nucleonics Laboratories, Inc., Bethesda, Maryland 20014

ABSTRACT

The method of preparing solid-phase immunosorbents by covalently attaching proteins from whole human serum to cyanogen bromide-activated agarose has been investigated to determine optimum concentrations of cyanogen bromide and protein, and the optimum pH for the maximum attachment of proteins from serum. Two systems in which the above immunosorbents have proved useful are described: the removal of antibodies to normal serum proteins from anti-hepatitis B serum and the removal of light chain antibodies from anti-human immunoglobulin M serum.

Appl Environ Microbiol. 1973 June; 25(6): 880-884
Copyright © 1973 American Society for Microbiology. All Rights Reserved.





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Copyright © 1973 by the American Society for Microbiology. All rights reserved.