AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Singh, B.
Right arrow Articles by Hammon, W. McD.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Singh, B.
Right arrow Articles by Hammon, W. McD.
Agricola
Right arrow Articles by Singh, B.
Right arrow Articles by Hammon, W. McD.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1973 June; 25(6): 945-951
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Semi-Commercial-Scale Production of Japanese B Encephalitis Virus Vaccines from Tissue Culture

Balwant Singh, Ik Chin Chang1 and W. McD. Hammon

a Department of Epidemiology and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania 15213

ABSTRACT

This study was undertaken to modify and develop procedures for tissue culture-inactivated Japanese B encephalitis (JBE) virus vaccine production in large quantities. Various types of glass bottles were tried and, considering many advantages, long cylindrical roller (CR) bottles were selected. Several variables were investigated including number and volume of trypsinized cells to be seeded, volume of growth medium required for optimum cell growth, amount of calf serum, and volume of harvest medium for a high-titer virus yield. A good confluent cell sheet in CR bottles was obtained within a week by increasing the calf serum from 4 to 10% and when such tissue in a CR bottle was inoculated with 45,000 viral mean tissue culture infective doses directly into the medium, the cytopathological effects (CPE) appeared on day 5. High-titer virus yields were obtained when the harvests were made at 4+ CPE using medium 199 with 2% human albumin at pH 8.3 to 8.5. No appreciable gain in titer was found from such harvests by blending to release intracellular virions. The production methods finally adopted gave consistently good results, and several inactivated JBE virus vaccine lots with minimum immunizing doses, ranging from 0.005 to 0.017 ml, were prepared using a large number of CR bottles in a simulated commercial-scale production system.

FOOTNOTES

1 Present address: Michigan Department of Public Health Laboratories, Lansing, Mich. 48924.

Appl Environ Microbiol. 1973 June; 25(6): 945-951
Copyright © 1973 American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1973 by the American Society for Microbiology. All rights reserved.