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Appl Environ Microbiol. 1973 June; 25(6): 952-955
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Identification of the Volatile Compounds Produced in Sterile Fish Muscle (Sebastes melanops) by Pseudomonas fragi¹

^a Department of Food Science and Technology, Oregon State University, Corvallis, Oregon 97331

ABSTRACT

Volatile compounds produced by Pseudomonas fragi strain 18 in sterile fish muscle (Sebastes melanops) were identified by combined gas-liquid chromatography and mass spectrometry. Compounds positively identified included dimethyl sulfide, acetaldehyde, ethyl acetate, ethyl alcohol, and dimethyl disulfide. Methyl mercaptan, ethyl butyrate, ethyl hexanoate, and butanone were tentatively identified by relative retention times of the authentic compounds. The fruity odor that developed in fish muscle during incipient spoilage was attributed to a synergistic flavor interaction involving the ethyl esters of acetate, butyrate, and hexanoate.

¹ Technical paper no. 3506, Oregon Agricultural Experiment Station, Oregon State University, Corvallis, Ore. 97331.