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Appl Environ Microbiol. 1973 June; 25(6): 981-990
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Identification of the Prototheca Species by Immunofluorescence

M. Sam Sudman1 and William Kaplan

Department of Parasitology and Laboratory Practice, School of Public Health, University of North Carolina, Chapel Hill, North Carolina 27514
Center for Disease Control, Health Services and Mental Health Administration, Public Health Service, U.S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333

ABSTRACT

Studies were carried out to develop fluorescent antibody reagents for the identification of the Prototheca species and for their differentiation from morphologically similar fungi of various genera in formalin-fixed tissues. Antisera against representative isolates of P. filamenta, P. moriformis, P. stagnora, P. wickerhamii, and P. zopfii were produced in rabbits. Antiglobulins, labeled with fluorescein-isothiocyanate that intensely stained most cells of the homologous species, were selected for use as potential diagnostic reagents. By adsorbing the conjugates with selected heterologous cross-staining protothecae, reagents that were both sensitive and specific were obtained. Evaluation of the adsorbed conjugates with sections of tissue infected with protothecae, sections of tissue infected with morphologically similar fungi, and cultures of protothecae showed that these reagents are useful for the rapid and reliable identification of the Prototheca species.

FOOTNOTES

1 Present address: Laboratory Improvement Division, Bureau of Laboratories, Michigan Department of Health, Lansing, Mich. 48914.

Appl Environ Microbiol. 1973 June; 25(6): 981-990
Copyright © 1973 American Society for Microbiology. All Rights Reserved.





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Copyright © 1973 by the American Society for Microbiology. All rights reserved.