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Appl Environ Microbiol. 1973 July; 26(1): 120-121
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Simplified Method for Producing Pyocins from Pseudomonas aeruginosa

Lois F. Jones, B. V. Pinto, Evan T. Thomas and J. J. Farmer III1

a Department of Microbiology, The University of Alabama, University, Alabama 35486

ABSTRACT

Good yields of pyocin are obtained when Pseudomonas aeruginosa is grown in Trypticase soy broth (without glucose) (BBL) plus 1% potassium nitrate. As a result, pyocin production for routine epidemiological typing can be done in screw-cap tubes, without mechanical agitation or mitomycin C induction.


FOOTNOTES

1 Present address: Bacteriophage Bacteriocin Laboratory, Enteric Unit, Center for Disease Control, Atlanta, Ga. 30333.


Appl Environ Microbiol. 1973 July; 26(1): 120-121
Copyright © 1973 American Society for Microbiology. All Rights Reserved.







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