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Appl Environ Microbiol. 1973 July; 26(1): 22-26
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Effect of Sodium Nitrite and Sodium Nitrate on Botulinal Toxin Production and Nitrosamine Formation in Wieners

Oscar Mayer and Company, Madison, Wisconsin 53706
Department of Bacteriology and Food Research Institute, University of Wisconsin, Madison, Wisconsin 53706
Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204
Division of Chemistry and Physics, Food and Drug Administration, Washington, D.C. 20204
Microbiology Staff, Meat and Poultry Inspection, U.S. Department of Agriculture, Washington, D.C. 20250
American Meat Institute Foundation, Chicago, Illinois 60605

ABSTRACT

Wieners were formulated and processed approximating commercial conditions as closely as possible. Twenty-four batches of product were made with the addition of six levels of sodium nitrite (0, 50, 100, 150, 200, and 300 µg/g), four levels of sodium nitrate (0, 50, 150, and 450 µg/g), and two levels of Clostridium botulinum (0 and 620 spores/g). After formulation, processing, and vacuum packaging, portions of each batch were incubated at 27 C or held for 21 days at 7 C followed by incubation at 27 C for 56 days. The latter storage condition approximated distribution of product through commercial channels and potential temperature abuse at the consumer level. Samples were analyzed for botulinal toxin, nitrite, and nitrate levels after 3, 7, 14, 21, 28, and 56 days of incubation. When nitrite was not added, toxic samples were detected after 14 days of incubation at 27 C. At the lowest level of nitrite added (50 µg/g), no toxic samples were observed until 56 days of incubation. Higher levels of nitrite completely inhibited toxin production throughout the incubation period. Nine uninoculated samples, representing various levels and combinations of nitrite and nitrate, were evaluated organoleptically. The flavor quality of wieners made with nitrite was judged significantly higher (P = 0.05) than of wieners made without nitrite. The nine samples were negative for 14 volatile nitrosamines at a sensitivity level of 10 ng/g. The results indicated that nitrite effectively inhibited botulinal toxin formation at commercially employed levels in wieners and that detectable quantities of nitrosamines were not produced during preparation and processing of the product for consumption.

¹ Present address: General Foods Corporation, Tarreytown, N.Y. 10591.