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Appl Environ Microbiol. 1973 August; 26(2): 219-220
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Rapid Method for Detection of Pseudomonas aeruginosa on MacConkey Agar Under Ultraviolet Light

Environmental Bacteriology Laboratory, Ontario Ministry of Health, Laboratory Services Branch, Box 9000, Terminal "A," Toronto, Ontario, Canada

ABSTRACT

A simple screening technique for the detection of Pseudomonas aeruginosa colonies by their fluorescence on MacConkey agar under ultraviolet light is proposed. From 306 nonlactose fermenting cultures screened under the ultraviolet light, 108 fluorescent isolates were obtained. These were screened biochemically, with 103 (94.8%) being verified as P. aeruginosa. From the 198 nonfluorescing cultures, only one suspected P. aeruginosa was isolated.