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Appl Environ Microbiol. 1973 September; 26(3): 386-390
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Microtissue Culture Plaque Assay for Herpesvirus saimiri

^a Human Tumor Studies Section, Viral Biology Branch, National Institutes of Health, Bethesda, Maryland 20014

ABSTRACT

A microtissue culture method for the plaque assay of Herpesvirus saimiri has been developed. Virus titrations carried out in Microtest II tissue culture plates (Falcon) yielded reproducible results that agreed well with those obtained by employing macrocultures. The described method is quantitative, reproducible, economical, and suitable for routine assay of large numbers of virus samples.

¹ Present address: Institute of Cancer Research, College of Physicians and Surgeons, Columbia University, New York, N.Y. 10032.