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Appl Environ Microbiol. 1973 September; 26(3): 426-428
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Pyridine Extraction of Nocardial Acid Fastness

B. L. Beaman and J. Burnside

Deapartment of Microbiology, Georgetown University Schools of Medicine and Dentistry, Washington, D.C. 20007

ABSTRACT

Several strains of Mycobacterium and Nocardia were stained for acid fastness by using the method of Kinyoun. Duplicate, glutaraldehyde-fixed smears were extracted with fresh pyridine prior to staining. The results clearly demonstrated that both in vivo- and in vitro-grown Nocardia differed from Mycobacterium by becoming nonacid fast after extraction. This pyridine extraction of nocardial acid fastness should have diagnostic and taxonomic application.

Appl Environ Microbiol. 1973 September; 26(3): 426-428
Copyright © 1973 American Society for Microbiology. All Rights Reserved.





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Copyright © 1973 by the American Society for Microbiology. All rights reserved.