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Appl Environ Microbiol. 1974 January; 27(1): 206-209
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

L-Asparaginase Production by the Rumen Anaerobe Vibrio succinogenes

¹ Department of Zoology and Physiology, Newark College of Arts and Sciences, Rutgers, The State University of New Jersey, Newark, New Jersey 07102

ABSTRACT

The rumen anaerobe Vibrio succinogenes possesses a constitutive L-asparaginase. The amount of enzyme produced is affected by the compound supplied to the organism to generate the fumaric acid it requires as a terminal electron acceptor. When nitrate is provided as the terminal electron acceptor, the amount of enzyme produced is affected by the compound provided to satisfy the nutritional requirement of the organism for succinic acid. Specific activities of up to 8.4 IU/mg of protein in cell-free extracts have been obtained. This specific activity is higher than has been previously reported for any organism. The enzyme has an apparent K_m of 1.7 x 10^-5 M and low activity towards L-glutamine when assayed at pH 8.5.