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Appl Environ Microbiol. 1974 January; 27(1): 38-42
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Medium Containing Trypan Blue and Antibiotics for the Detection of Cryptococcus neoformans in Clinical Samples

Richard M. Vickers, James J. McElligott Jr., John D. Rihs and Bosko Postic

The Laboratory and Medical Services of the Veterans Administration Hospital, Pittsburgh, Pennsylvania 15240
Department of Pathology (Microbiology Section), University of Pittsburgh, School of Medicine, Pittsburgh, Pennsylvania 15261
Department of Medicine, University of Pittsburgh, School of Medicine, Pittsburgh, Pennsylvania 15261

ABSTRACT

A medium containing trypan blue, gentamicin, and chloramphenicol is introduced for the detection of Cryptococcus neoformans and Cryptococcus species from clinical samples. Ten recently isolated strains of Cryptococcus species as well as several clinical isolates of C. neoformans incorporated trypan blue and produced dark blue colonies on this mycological medium, whereas other common yeasts were light blue. The laboratory diagnosis of two cases of cryptococcosis was accomplished by the isolation of C. neoformans on the antibiotic-dye-containing medium. Compared to conventional media supporting large numbers of Pseudomonas aeruginosa and other gram-negative bacilli, the new medium was selective for yeasts. In one instance, the colonization of the respiratory tract by C. neoformans which led to fungemia was traced by the use of the antibiotic-dye medium. The antibiotic mixture, utilized herein, was more effective in suppressing bacteria contained in samples from patients than a medium containing cycloheximide and chloramphenicol.

Appl Environ Microbiol. 1974 January; 27(1): 38-42
Copyright © 1974 American Society for Microbiology. All Rights Reserved.





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Copyright © 1974 by the American Society for Microbiology. All rights reserved.