AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ward, O. P.
Right arrow Articles by Fogarty, W. M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Ward, O. P.
Right arrow Articles by Fogarty, W. M.
Agricola
Right arrow Articles by Ward, O. P.
Right arrow Articles by Fogarty, W. M.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1974 February; 27(2): 346-350
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Polygalacturonate Lyase Production by Bacillus subtilis and Flavobacterium pectinovorum

Owen P. Ward and William M. Fogarty

Department of Industrial Microbiology, University College, Ardmore House, Dublin 4, Ireland

ABSTRACT

Nutritional factors relating to the production of polygalacturonate lyases by strains of Bacillus subtilis and Flavobacterium pectinovorum were examined. Studies were carried out in shake flask cultures. In the case of B. subtilis the enzyme was produced constitutively, whereas in the case of F. pectinovorum it was only produced in quantity in the presence of pectic substances. Glucose was the most suitable carbon source for production of the polygalacturonate lyase of B. subtilis; of the nitrogen sources examined, the highest activities per milliliter of supernatant and per milligram of cells were obtained with glutamine and ammonium sulfate, respectively. The pattern of enzyme production and growth was similar although enzyme production ceased at pH 5.3. Sodium polypectate was the best inducer of polygalacturonate lyase with F. pectinovorum. Highest activity per milliliter of cell-free supernatant was obtained with skin milk powder as nitrogen source, although ammonium sulfate gave highest enzyme production per unit of biomass. Growth of F. pectinovorum occurred between pH 5.7 and 7.2. Enzyme production occurred during active growth and was independent of the pH of the medium.

Appl Environ Microbiol. 1974 February; 27(2): 346-350
Copyright © 1974 American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1974 by the American Society for Microbiology. All rights reserved.