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Appl Environ Microbiol. 1974 February; 27(2): 429-431
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Stress: a Factor to be Considered in Heterotrophic Microorganism Enumeration from Aquatic Environments

Donald A. Klein and Shenyuh Wu

1 Department of Microbiology, Colorado State University, Fort Collins, Colorado 80521

ABSTRACT

Heterotrophic microorganisms in water samples are susceptible to the transient stress of warmed agar used in the standard methods pour plate procedure, causing significantly decreased recoveries in comparison with a spread plate technique. Microbial starvation can increase susceptibility to a transient warming stress. The standard plate count procedure, as presently described, should not be considered for quantitation of microorganisms from aquatic environments.

Appl Environ Microbiol. 1974 February; 27(2): 429-431
Copyright © 1974 American Society for Microbiology. All Rights Reserved.





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Copyright © 1974 by the American Society for Microbiology. All rights reserved.