This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Smaron, M. F. Articles by Vice, J. L. Search for Related Content PubMed Articles by Smaron, M. F. Articles by Vice, J. L. Agricola Articles by Smaron, M. F. Articles by Vice, J. L.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1974 March; 27(3): 469-474
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Analysis of Corynebacterium vaginale by an Immunodiffusion Technique

Department of Pathology, Loyola University Medical Center, Maywood, Illinois 60153
Department of Microbiology, Loyola University Medical Center, Maywood, Illinois 60153

ABSTRACT

An immunodiffusion technique was employed to study the antigenic relationship of Corynebacterium vaginale isolates, vaginal diphtheroids, and members of the genera Corynebacterium and Lactobacillus. Antisera were prepared against C. vaginale ATCC strain 14018 grown diphasically and on blood agar plates and were tested against extracts of organisms prepared by sonication. Ouchterlony analysis demonstrated that all of the isolates of C. vaginale examined possess a common antigenic determinant. No antigenic relationship was detected between C. vaginale and members of the genera Corynebacterium or Lactobacillus. This study also demonstrated that alterations in the cultural conditions can cause variations in the antigenic composition of C. vaginale.