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Appl Environ Microbiol. 1974 March; 27(3): 480-483
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Tryptic Soy Bile-Kanamycin Test for the Identification of Bacteroides fragilis

¹ Anaerobe Laboratory, Department of Microbiology and Immunology, School of Medicine, Temple University Health Sciences Center, Philadelphia, Pennsylvania 19140

ABSTRACT

A simple and practical test for the identification of Bacteroides fragilis is described. It utilizes two well-known properties of this species, i.e., stimulation of growth by bile and resistance to kanamycin. The test media are a tryptic-soy bile agar plate and a supplemented blood agar plate on which a kanamycin 1,000-µg/ml disk is placed. Incubation is for 24 h at 37 C in GasPak. The results of screening 190 strains, mostly clinical isolates, indicate that B. fragilis can be easily and reliably distinguished from other Bacteroides and from Fusobacterium species by its growth on tryptic-soy bile agar and resistance to kanamycin.