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Appl Environ Microbiol. 1974 March; 27(3): 531-536
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Production of Flavine-Adenine Dinucleotide from Riboflavine by a Mutant of Sarcina lutea

Department of Biochemistry, Research Labolatory of Applied Biochemistry, Tababe Seiyaku Co., Ltd., 962 Kashima-cho, Higashiyodogawa-ku, Osaka, Japan

ABSTRACT

A study was made to develop a new method for the production of flavine-adenine dinucleotide (FAD) from riboflavine and adenine by a mutant of Sarcina lutea deficient in the enzyme adenosine deaminase. It was found that this strain could convert exogenously supplemented riboflavine to extracellular FAD. The yields of FAD were increased by addition of D-cycloserine in the culture medium. The culture conditions for FAD production were investigated under the addition of D-cycloserine, and increased production of FAD was observed with the addition of an appropriate amount of thiamine, acetate, and sodium ion. The yield of 0.7 g/liter was obtained in the optimal culture in 5 days. Accumulated FAD was readily isolated by adsorption chromatography and ion-exchange chromatography in a 70% yield.