AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dhillon, E. K. S.
Right arrow Articles by Dhillon, T. S.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Dhillon, E. K. S.
Right arrow Articles by Dhillon, T. S.
Agricola
Right arrow Articles by Dhillon, E. K. S.
Right arrow Articles by Dhillon, T. S.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1974 April; 27(4): 640-647
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Synthesis of Indicator Strains and Density of Ribonucleic Acid-Containing Coliphages in Sewage

Elvera K. S. Dhillon and T. S. Dhillon

Biology Department, The Chinese University of Hong Kong, N.T., Hong Kong
Department of Botany, University of Hong Kong, Hong Kong

ABSTRACT

Escherichia coli strains freshly isolated from natural sources are inefficient indicators of coliphages present in sewage. Four E. coli strains recently isolated from clinical specimens were mutagenized to obtain lac- mutants. Such mutants were infected with an F'lac+ sex factor of E. coli K-12. Pairs of isogenic lac- and lac-/F'lac+ strains were used as indicators of coliphages present in sewage, and it was found that such strains can be effectively used for a direct and almost selective enumeration of F-specific coliphage contents of sewage samples. Serological tests were applied to a number of F-specific phages isolated. All the isolates that were tested fell into two distinguishable antigenic classes: members of one class being related to ribonucleic acid (RNA) phage MS2 and those of the other being related to another RNA phage, namely, Qß. MS2-related phages have been found to be more widely distributed than the Qß related phages. Most habitats sampled were found to yield only one or the other kind of phage. Single-stranded deoxyribonucleic acid-containing F-specific phages were not detectable by the methods employed by us.

Appl Environ Microbiol. 1974 April; 27(4): 640-647
Copyright © 1974 American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1974 by the American Society for Microbiology. All rights reserved.