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Appl Environ Microbiol. 1974 April; 27(4): 699-702
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Comparison of Two Liquid Blood Culture Media Containing Sodium Polyanetholesulfonate: Tryptic Soy and Columbia

Mayo Clinic, Rochester, Minnesota 55901
Mayo Foundation, Rochester, Minnesota 55901

ABSTRACT

In a comparison of tryptic soy broth and Columbia broth, two blood culture media containing sodium polyanetholesulfonate, there were 589 positive cultures (excluding presumed contaminants). The two media were equivalent in performance except for lower detection rates for Staphylococcus aureus (P < 0.01) and Pseudomonas aeruginosa (P = 0.05) and a higher detection rate for Bacillus (P < 0.01) in Columbia broth. No significant differences were noted in time intervals to detection of positivity. Routine subcultures on the 1st and 5th days of incubation provided the initial detection of 18.1% of the positive cultures.