This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Tosa, T. Articles by Chibata, I. Search for Related Content PubMed Articles by Tosa, T. Articles by Chibata, I. Agricola Articles by Tosa, T. Articles by Chibata, I.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1974 May; 27(5): 886-889
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Basic Studies for Continuous Production of L-Aspartic Acid by Immobilized Escherichia coli Cells

Department of Biochemistry, Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd., Kashima-cho, Higashiyodogawa-ku, Osaka, Japan

ABSTRACT

By using a column packed with immobilized Escherichia coli cells entrapped in a polyacrylamide gel lattice, conditions for continuous production of L-aspartic acid from ammonium fumarate were investigated. When a solution of 1 M ammonium fumarate (pH 8.5) containing 1 mM Mg²⁺ was passed through the immobilized cell column at a flow rate of space velocity (SV) = 0.8 at 37 C, the highest rate of reaction was attained. From the column effluents, L-aspartic acid was obtained in good yield. The immobilized cell column was very stable.