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Appl Environ Microbiol. 1974 May; 27(5): 985-987
Copyright © 1974 American Society for Microbiology. All Rights Reserved.
A Serum Bottle Modification of the Hungate Technique for Cultivating Obligate Anaerobes
Terry L. Miller and
M. J. Wolin
Department of Dairy Science, University of Illinois, Urbana, Illinois 61801
Department of Microbiology, University of Illinois, Urbana, Illinois 61801
ABSTRACT
The modification described utilizes a serum bottle closed with a butyl rubber stopper with a crimped metal seal for the growth of obligately anaerobic bacteria.
Appl Environ Microbiol. 1974 May; 27(5): 985-987
Copyright © 1974 American Society for Microbiology. All Rights Reserved.
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Copyright © 1974 by the American Society for Microbiology. All rights reserved.