This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Nickerson, K. W. Articles by Bulla, L. A. Search for Related Content PubMed Articles by Nickerson, K. W. Articles by Bulla, L. A., Jr. Agricola Articles by Nickerson, K. W. Articles by Bulla, L. A.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1974 July; 28(1): 124-128
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Physiology of Sporeforming Bacteria Associated with Insects: Minimal Nutritional Requirements for Growth, Sporulation, and Parasporal Crystal Formation of Bacillus thuringiensis

Northern Regional Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604

ABSTRACT

A defined medium is described in which 18 strains of Bacillus thuringiensis representing the 12 established serotypes grow, sporulate, and produce a parasporal crystal. This minimal medium contains glucose and salts supplemented with either aspartate, glutamate, or citrate. These organic acids are required and cannot be replaced by vitamin mixtures or succinate even though succinate is taken up at a rate similar to that of aspartate, glutamate, and citrate.

¹ Present address: Department of Plant Pathology, University of Nebraska, Lincoln, Nebr. 68503.

² Present address: U.S. Grain Marketing Research Center, 1515 College Ave., Manhattan, Kans. 66502.