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Appl Environ Microbiol. 1974 July; 28(1): 86-90
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Comparison of Purified Alpha-Toxins from Various Strains of Staphylococcus aureus

Department of Microbiology, The University of Georgia, Athens, Georgia 30602

ABSTRACT

Alpha-toxin from five strains of Staphylococcus aureus, including Wood 46, was purified by isoelectric focusing. The alpha-toxins obtained from different strains were similar. The isoelectric point of the purified toxins was 8.65 ± 0.15. Sharp concentration peaks were not always obtained. In the ultracentrifuge the alpha-toxins migrated usually as three peaks which could be dissociated with propionic acid to yield one peak. A single line of identity was obtained in immunoelectrophoresis when a heterologous antiserum was reacted with the five purified toxins. It was concluded that the widespread use of the Wood 46 strain for the production of alpha-toxin is justified.

¹ Present address: Hoffman LaRoche Inc., Nutley, N. J. 07110.