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Appl Environ Microbiol. 1974 July; 28(1): 91-94
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Use of Sodium Polyanethol Sulfonate in the Preparation of 5% Sheep Blood Agar Plates

Departments of Pathology and Microbiology, Bowman Gray School of Medicine, and Clinical Microbiology Laboratory, North Carolina Baptist Hospital, Winston-Salem, North Carolina 27103

ABSTRACT

An alternative method to defibrinating sheep blood for use in bacteriological media is described. The new procedure incorporates sodium polyanethol sulfonate in a concentration of 0.05% (vol/vol). In testing 117 bacterial and fungal isolates, no significant differences were found with respect to adequate growth, pigment production, hemolytic reactions, and other physical attributes. Further tests demonstrate that the sodium polyanethol sulfonate in sheep blood agar plates does not cause any aberrations in zone sizes around disks used in antibiotic susceptibility tests. Consequently, the method represents a suitable alternative to the use of defibrinated sheep blood in the preparation of bacteriological media.