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Appl Environ Microbiol. 1974 August; 28(2): 226-231
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Simplified Approach to Identification of Aerobic Actinomycetes by Thin-Layer Chromatography

Section of Clinical Microbiology, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55901

ABSTRACT

A system has been developed for the identification of aerobic actinomycetes in the clinical laboratory based on analysis of whole cells for diaminopimelic acid and carbohydrates and on the ability of the organism to decompose casein, tyrosine, and xanthine media. The whole-cell analyses were performed by a simple thin-layer chromatographic procedure that is described. Eighteen reference cultures were correctly identified and, subsequently, 35 isolates from clinical material were grouped by using this system. The method is well suited for use in routine clinical laboratories.

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