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Appl Environ Microbiol. 1974 August; 28(2): 239-244
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Modified Radioimmunoassay for Murine Sarcoma-Leukemia Virus Group-Specific Antigen

Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas 77025

ABSTRACT

Iodination of disrupted Moloney strain murine sarcoma-leukemia virus resulted in labeled group-specific (gs) protein which was subsequently purified on an isoelectrofocusing column. This iodinated purified gs antigen, prepared from a relatively small quantity of purified virus, was used in a radioimmunoassay. A radioimmunoassay inhibition method was developed so that antibody specific for mammalian C-type gs antigen could be measured in undiluted or low dilutions of test serum without altering the known reagents of the test. The gs antigen isolated from purified Moloney strain murine sarcoma-leukemia virus has an isoelectric point (pH 5.95) which is significantly lower than that reported for other murine leukemia viruses.