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Appl Environ Microbiol. 1974 August; 28(2): 271-275
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Trichophytin Extraction: Biological Comparison of Trichophytin Extracted from Trichophyton mentagrophytes Grown in a Complex Medium and a Defined Medium

Dermatology Division, Letterman Army Institute of Research, Presidio of San Francisco, San Francisco, California 94129

ABSTRACT

The extraction of antigens (trichophytin) from Trichophyton mentagrophytes var. asteroides grown in both a complex medium and a defined medium proved to be reproducible and feasible. These extracts were first evaluated by skin testing in both infected and noninfected guinea pigs. Further evaluation and standardization was accomplished in selected humans by comparing these antigens to a trichophytin known to elicit allergic reactions to the dermatophytes. The trichophytins produced by this extraction procedure gave no false positive (or negative) skin test reactions. These antigens did, however, reliably elicit allergic sensitivity of both the immediate and delayed type. The potency of both complex and defined media antigen was shown to be equal to that of the standard trichophytin.

¹ Present address: Department of Health and Environmental Control Laboratories, Columbia, S.C. 29201.

² Present address: Department of Dermatology, University of Michigan Medical Center, Ann Arbor, Mich. 48104.