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Appl Environ Microbiol. 1974 September; 28(3): 340-341
Copyright © 1974 American Society for Microbiology. All Rights Reserved.
Department of Bacteriology and Biochemistry, University of Victoria, Victoria, British Columbia, Canada
ABSTRACT
The ventral surface of spawning salmonid fish was opened aseptically and the roe were removed aseptically. Roe obtained by using this technique were demonstrated to be sterile. Aseptic fertilization and incubation of eggs obtained in this manner resulted in the hatching of gnotobiotic salmonid fish.
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