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Appl Environ Microbiol. 1974 September; 28(3): 347-350
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Rice-Grown Rhizopus oligosporus Inoculum for Tempeh Fermentation

Laboratorium Mikrobiologi, Institut Teknologi Bandung, Bandung, Indonesia

ABSTRACT

A method of growing Rhizopus oligosporus on cooked rice as the inoculum for the fermentation of soybeans into tempeh was described and evaluated. Isolated R. oligosporus spores on glass beads survived best at low temperature and intermediate humidity. The activity of the rice-grown inoculum to ferment soybeans into tempeh did not decrease appreciably when stored desiccated for one year at 4 C or room temperature. Bacterial contaminants as high as 10⁸ counts per g of cooked soybeans did not seem to affect the fermentation.

¹ Present address: School of Biological Sciences, University of Kentucky, Lexington, Ky. 40506.

² Present address: Food Science Department, Agricultural University, Wageningen, The Netherlands.