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Appl Environ Microbiol. 1974 September; 28(3): 423-430
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

8-Classtype Tallying Device and Counter

Department of Microbiology, School of Medicine, University of South Dakota, Vermillion, South Dakota 57069

ABSTRACT

An electronic device using transistor-transistor-logic integrated circuits as primary building blocks was designed and constructed to accumulate counts for eight classtypes. Selection of the classtypes is provided for by three encoders, X, Y, and Z. Using these encoders allows selection of memory counters for accumulations of 0 to 99 counts per classtype. Making X, Y, and Z the input equivalents of three genes permitted direct tallying and accumulation of totals of individual phenotypes for recombinant progeny containing three scorable genes. The use of the device as a simple one-of-four colony counter is described. Use of the instrument for obtaining counts of multiply characterized colonies was possible. In all tested instances, tedium and the labor of obtaining differential counts was reduced by more than fourfold with a great increase in accuracy of count accumulations. Complete information is provided for the fabrication of the instrument for use in individual laboratories.