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Appl Environ Microbiol. 1974 September; 28(3): 456-459
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Ice Nucleation Induced by Pseudomonas syringae1

Leroy R. Maki, Elizabeth L. Galyan, Mei-Mon Chang-Chien and Daniel R. Caldwell

Division of Microbiology and Veterinary Medicine, University of Wyoming, Laramie, Wyoming 82071

ABSTRACT

Broth cultures of suspensions of Pseudomonas syringae isolated from decaying alder leaves (Alnus tenuifolia) were found to freeze at very warm (-1.8 to -3.8 C) temperatures. The initiation of freezing appears associated with the intact cell and not with extracellular material. Chemical treatments and physical destruction of the cell destroy activity. Bacteria must be in concentrations of approximately 106/ml before freezing at warm temperatures occurs.


FOOTNOTES

1 Wyoming Agricultural Experiment Station Journal article no. 673.


Appl Environ Microbiol. 1974 September; 28(3): 456-459
Copyright © 1974 American Society for Microbiology. All Rights Reserved.







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Copyright © 1974 by the American Society for Microbiology. All rights reserved.