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Appl Environ Microbiol. 1974 October; 28(4): 553-556
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Microadaption of Hemadsorption Inhibition for Neutralization Tests with Pig Hemagglutinating Encephalomyelitis Virus

K. J. Sørensen

State Veterinary Institute for Virus Research, Lindholm, DK-4771 Kalvehave, Denmark

ABSTRACT

A microneutralization test is described, in which secondary pig thyroid cells are used and end points are determined by hemadsorption with hamster erythrocytes. The method is evaluated and compared with a macromethod, in which the fluorescent-antibody staining technique is applied for reading titers. The micromethod was found to be useful because of its sensitivity and rapidity.

Appl Environ Microbiol. 1974 October; 28(4): 553-556
Copyright © 1974 American Society for Microbiology. All Rights Reserved.





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Copyright © 1974 by the American Society for Microbiology. All rights reserved.