This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Mackowiak, P. A. Articles by Huq, I. Search for Related Content PubMed Articles by Mackowiak, P. A. Articles by Huq, I. Agricola Articles by Mackowiak, P. A. Articles by Huq, I.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1974 October; 28(4): 586-588
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Rapid Method of Determining Cholera Vibrio Biotype

Cholera Research Laboratory, Dacca, Bangladesh

ABSTRACT

The characteristic motility of cholera vibrios, as viewed through a dark-field microscope, and the adhesiveness of chicken cell-positive vibrios provide a means for rapidly identifying and biotyping cholera vibrios. Dilute suspensions of vibrios, such as one might find in a fresh rectal swab specimen from a cholera patient, when mixed with a 0.25% suspension of chicken erythrocytes in saline, can be used to biotype the cholera vibrios without prior isolation in pure culture. This is accomplished by using a dark-field microscope through which the chicken cell-positive cholera vibrios are observed to attach to the scattered erythrocytes and to propel them with a characteristic flipping motion.

¹ Present address: Veterans Administration Hospital, Dallas, Tex. 75216.