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Appl Environ Microbiol. 1974 October; 28(4): 618-622
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Identification of Salmonella with the O-1 Bacteriophage

Department of Immunology and Medical Microbiology, University of Florida, College of Medicine, Gainesville, Florida, and the Bureau of Laboratories, Florida State Board of Health, Jacksonville, Florida

ABSTRACT

The O-1 bacteriophage test of Cherry et al. (1954) for the presumptive identification of salmonellae in the diagnostic laboratory was investigated. A phage lysate with a titer of 10¹² plaque-forming units per ml was found to be optimal. This preparation lysed 98.2% of Salmonella strains tested, while maintaining its high specificity for salmonellae. Gram-negative organisms other than salmonellae were resistant to the O-1 phage; however, 5.9% of Escherichia coli strains tested were susceptible. The O-1 phage test is a simple, rapid, inexpensive, sensitive, and specific procedure for the identification of salmonellae in the diagnostic laboratory. A presumptive identification is obtained 1 day earlier than with conventional biochemical tests.