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Appl Environ Microbiol. 1974 October; 28(4): 668-671
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Evaluation of the Minitek System for Identification of Enterobacteriaceae

Diagnostic Microbiology Service, Columbia-Presbyterian Medical Center; and the Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032

ABSTRACT

Clinical isolates (869) and stock cultures (35) of Enterobacteriaceae were tested in parallel with the Minitek and conventional systems. The Minitek correctly identified 822 of 904 cultures. When a deoxyribonuclease plate was inoculated along with the Minitek, it was possible to speciate Enterobacteriaceae within 24 h. False-positive hydrogen sulfide reactions were the major fault with this system. Reactions were clear-cut and easy for technologists to read.